Brian Markinson and John Pyper-Ferguson had both been in the previous Battlestar Galactica prequel Caprica. John Pyper-Ferguson was also in Battlestar Galactica.
Tricia Helfer vocally reprises her role of Number Six and recycles both her first line of "Are you alive?" and snapping someone's neck from Battlestar Galactica.
Many of the crew members on the Osiris were played by actors who played recurring characters on Battlestar Galactica. Jill Teed (Commander Ozar) was Sergeant Hadrian, Ty Olsson (Helmsman) was Captain Aaron Kelly, and Sebastian Spence (Lt. Jim Kirby) was Lt. Noel 'Narcho' Allison.